Pet Nutrition Research Department, Nestle Purina Pet Care Co, Checkerboard Square, St Louis, MO 63164. (Kealy, Lawler, Mantz); Statistical Services Department, Nestle Purina Pet Care Co, Checkerboard Square, St Louis, MO 63164. (Ballam); Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104.

(Biery, Smith); Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801. (Greeley, Segre); James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. (Lust); Department of Large Animal Clinical Medicine, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48823. Objective—To evaluate the effects of 25% diet restriction on life span of dogs and on markers of aging. Design—Paired feeding study. Turtle odyssey 2 free download for android windows 7.

Animals—48 Labrador Retrievers. Procedures—Dogs were paired, and 1 dog in each pair was fed 25% less food than its pair-mate from 8 weeks of age until death.

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Serum biochemical analyses were performed, body condition was scored, and body composition was measured annually until 12 years of age. Age at onset of chronic disease and median (age when 50% of the dogs were deceased) and maximum (age when 90% of the dogs were deceased) life spans were evaluated.

Results—Compared with control dogs, food-restricted dogs weighed less and had lower body fat content and lower serum triglycerides, triiodothyronine, insulin, and glucose concentrations. Median life span was significantly longer for dogs in which food was restricted.

The onset of clinical signs of chronic disease generally was delayed for food-restricted dogs. Conclusions and Clinical Relevance—Results suggest that 25% restriction in food intake increased median life span and delayed the onset of signs of chronic disease in these dogs. ( J Am Vet Med Assoc 2002;220:1315–1320).

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